Informatics Publishing Limited

D. S. Pokharkar ¹, R. V. Nakat ¹, N. D. Tamboli ¹, A. S. Dhane ¹, S. S. Jadhav ¹, A. G. Chandele ¹, R. J. Rabindra ²

Affiliations
1 All India Co-ordinated Research Project on Biological Control of Crop Pests and Weeds, Mahatma Phule Krishi Vidyapeeth, College of Agriculture, Pune 411 005, Maharashtra, IN
2 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore 560024, Karnataka, IN

Abstract

Papaya ( Carica papaya L.) is grown over 2300 ha in Maharashtra and almost 50% of the crop is utilized for papain production. Recently, the papaya crop was found to be infested by the invasive papaya mealybug (PMB), Paracoccus marginatus Williams and Granara de Willink. The PMB was first noticed in Maharashtra in the papaya orchard at Regional Fruit Research Station (RFRS), Ganeshkhind, Pune on 14 ^th July, 2010 by a survey team of scientists of the All India Co-ordinated Research Programme on Biological Control of Crop Pests and Weeds, Mahatma Phule Krishi Vidyapeeth, College of Agriculture, Pune.

Full Text

   

S. D. Patil ¹, J. R. Kadam ², A. G. Chandele ², S. S. Wagh ², R. S. Jadhav ²

Affiliations
1 Department of Entomology, Agricultural Research Station, Niphad, Nasik (M.S.), IN
2 Department of Entomology, Mahatma Phule Krishi Vidhyapeeth, Rahuri, Ahmednagar (M. S.), IN

Abstract

The entomopathogenic fungus, Metarhizium anisopliae (Metschinikoff) Sorokin was mass produced in different liquid media. The nine media of various nutrient sources were evaluated to find out the most suitable medium for growth, biomass and viability of M.anisopliae. Sabouraud's dextrose broth with yeast extract proved to be the superior which gave significantly highest cfu (12.33x108/ml) and biomass (7.20g). The next best medium was Sabouraud's maltose broth with yeast extract and potato dextrose broth with yeast extract which registering cfu count of (10.33 × 108 and 10.67 × 108cfu/ml) and biomass (6.27 and 5.73 g), respectively. The lowest (48.33%) medium surface coverage and least biomass (1.57g) and cfu (4.33 × 108/ ml) were registered in medium with malt extract. Thus, considering growth, development and viability of M.anisopliae, Sabouraud's dextrose broth with yeast extract (SDY) emerged as the most potential medium for biomass production.

Keywords

Metarhizium Anisopliae, Yeast Extract, Biomass, Colony Forming Unit

Full Text

     

P. Nahar ¹, P. Yadav ¹, M. Kulye ¹, A. Hadapad ¹, M. Hassani ², U. Tuort ², S. Keller ³, A. G. Chandele ⁴, B. Thomas ², M. V. Deshpande ¹

Affiliations
1 Biochemical Sciences Division, National Chemical Laboratory, Pune 411 008, IN
2 Atlas Agro Binzstr., 18 CH-8045, Zurich, CH
3 Swiss Federal Research Station for Agroecology and Agriculture, FAL Postfach 8046, Zurich Rcckenholz, CH
4 Department of Entomology. College of Agriculture, Pune 411 005,, IN

Abstract

The effectiveness of oil-based conidia formulations of indigenous fungal isolates Metarhizillm anisopliae M34412, Beallveria bassiana 83301 and Nomuraea rileyi N812 were evaluated against Helicoverpa armigera (Hubner) infestation on pigeon pea under field conditions. The M. allisopliae M34412 conidia in the oil formulation (7:3, diesel: Sunflower oil) were found to be most effective in controlling H. armigera. The results were compared with other control agents such as, endosulfan and HaNPV (H. armigera Nuclear Polyhedrosis Virus). The per cent efficacies were, .W. anisopliae,/i> 66.74, endosulfan 62.58, N. rileyi 60.88 and HaNPV 55.58. B. bassiana preparation was found to be relatively less effective (51.25% efficacy). The effectiveness of all the control agents based on per cent pod damage and yield has also been discussed.

Keywords

Beauveria bassiana, Helicoverpa amligera, Metarlzizium anisopliae, Nomuraea Rileyi, Indigenous Fungal Isolates.

Full Text

   

P. N. Magar ¹, S. R. Kulkarni ², A. G. Chandele ²

Affiliations
1 Department of Entomology, Post Graduate Institute, Mahatma Phule Krishi Vidyapeeth, Rahuri, Dist. Ahmednagar - 413 722, Maharastra, IN
2 Department of Entomology, Post Graduate Institute, Mahatma Phule Krishi Vidyapeeth, Rahuri, Dist. Ahmednagar - 413 722, Maharastra, IN

Abstract

Fruit piercing moths, Eudocima sp. (Noctuidae) are common pests of ripening fruit in tropical and subtropical Southeast Asia, Australia, and the western Pacific islands. Surveys were conducted in Maharashtra revealed that three abundant larval parasitoids viz., a tachinid fly, Gonipthalmus hali, eulophid wasps, Euplectrus maternus and Tetrastichus sp. were contributing up to 36.65, 41.46 and 36.58 % larval mortality, respectively. The host specificity of parasitoids was studied in the laboratory by exposing them to larvae of Eudocima sp. However, Goniopthalmus hali showed parasitization on other species of Eudocima, whereas E. maternus is found to be host specific on E. materna species. In laboratory investigation, 72 to 89% parasitization of Trichogramma chilonis on the eggs of E. materna was also recorded.

Keywords

Eudocima Spp., Fruit Piercing Moth, Larval Parasitoids.

Full Text